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Calcitonin receptor binding affinity and in vitro bioactivity of mAb-sCT...
Calcitonin receptor binding affinity and in vitro bioactivity of mAb-sCT determined by using intracellular cAMP stimulation in human T47D cells. 50,000 cells/well were cultured for 2 days in 48-well...
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In vitro cytotoxicity of mAb, mAb-sCT & sCT on RAW 264.7 cells was...
In vitro cytotoxicity of mAb, mAb-sCT & sCT on RAW 264.7 cells was determined by MTT assay. No demonstrable cytotoxicity was seen as measured by the absorbance of formazan solution formed after 4...
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Demonstration of resorption pit formation on osteologic substrate plate....
Demonstration of resorption pit formation on osteologic substrate plate. After 7 days of culture, adherent cells were removed, and integrated density value of the remaining osteologic layer was...
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Tartrate-resistant acid phosphatase (TRAP) activity assay. mAb-sCT conjugate...
Tartrate-resistant acid phosphatase (TRAP) activity assay. mAb-sCT conjugate showed an inhibitory effect on TRAP activity, an important marker of osteoclasts. RAW: cells cultured in media with 0 ng/ml...
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(a) Confocal images of immunocytochemistry performed with anti-RANK mAb...
(a) Confocal images of immunocytochemistry performed with anti-RANK mAb which detected RANK receptors, the surface receptors present on the osteoclasts as well as osteoclast precursors....
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Multinucleated TRAP-positive osteoclast was observed by TRAP staining. RAW...
Multinucleated TRAP-positive osteoclast was observed by TRAP staining. RAW 264.7cells, a murine macrophage-like osteoclast precursor line, were stimulated with 50 ng/ml RANKL and 25 ng/ml MCSF for...
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Western Blot Analysis: Lane 1: a band seen between 20 kDa and 15 kDa as a...
Western Blot Analysis: Lane 1: a band seen between 20 kDa and 15 kDa as a result of chemiluminescent detection of anti-RANK IgG. Lane 2: standard marker. RANK receptor (19.3 kDa) was electrophoresed...
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(a) ELISA conducted on RANK receptor-coated plate. The optical density was...
(a) ELISA conducted on RANK receptor-coated plate. The optical density was seen to be proportional with the concentration of anti-RANK antibody. (b) ELISA of the mAb-sCT conjugate where...
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SDS-PAGE analysis of mAb-sCT conjugate and unconjugated antibody. A protein...
SDS-PAGE analysis of mAb-sCT conjugate and unconjugated antibody. A protein band above 150 kDa was seen for mAb-sCT conjugate in non-reduced SDS-PAGE analysis. Some bands above 50 kDa as well as 25...
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(a) Thiolation of antibodies using Traut’s reagent. (b) Synthesis of thiol...
(a) Thiolation of antibodies using Traut’s reagent. (b) Synthesis of thiol reactive sCT analogue by reacting sCT with NHS-PEG-MAL. c Coupling free thiol containing mAb with functionalized thiol...
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